Difference between revisions of "Part:BBa K4830029:Design"

 
 
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
 
The eGFP_complex premature stop codon requires a specific mutation to the wild-type eGFP.
 
The eGFP_complex premature stop codon requires a specific mutation to the wild-type eGFP.
−
 
−
 
  
 
===Source===
 
===Source===
−
 
 
The eGFP_complex premature stop codon is derived from the wild-type eGFP.
 
The eGFP_complex premature stop codon is derived from the wild-type eGFP.
 
The wild-type eGFP is derived from Aequorea victoria. It has an excitation maxima of 488 nm and emission maxima of 509 nm.
 
The wild-type eGFP is derived from Aequorea victoria. It has an excitation maxima of 488 nm and emission maxima of 509 nm.
  
 
===References===
 
===References===
 +
Certo, M. T., Ryu, B. Y., Annis, J. E., Garibov, M., Jarjour, J., Rawlings, D. J., & Scharenberg, A. M. (2011). Tracking genome engineering outcome at individual DNA breakpoints. Nature Methods, 8(8), 671–676.

Latest revision as of 17:54, 11 October 2023


eGFP_complex premature stop codon


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 192
    Illegal PstI site found at 160
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 192
    Illegal PstI site found at 160
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 192
    Illegal PstI site found at 160
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 192
    Illegal PstI site found at 160
    Illegal NgoMIV site found at 734
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The eGFP_complex premature stop codon requires a specific mutation to the wild-type eGFP.

Source

The eGFP_complex premature stop codon is derived from the wild-type eGFP. The wild-type eGFP is derived from Aequorea victoria. It has an excitation maxima of 488 nm and emission maxima of 509 nm.

References

Certo, M. T., Ryu, B. Y., Annis, J. E., Garibov, M., Jarjour, J., Rawlings, D. J., & Scharenberg, A. M. (2011). Tracking genome engineering outcome at individual DNA breakpoints. Nature Methods, 8(8), 671–676.