Difference between revisions of "Part:BBa K4645005"

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We assembled Pqtip with <bbpart>BBa_K4645004</bbpart>then transfected into E. coli to sensing the density of bacteria and regulate the circuit.</P>
 
We assembled Pqtip with <bbpart>BBa_K4645004</bbpart>then transfected into E. coli to sensing the density of bacteria and regulate the circuit.</P>
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===Characterization===
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<p>We inserted cCFP reporter gene behind qitp promoter to verify whether qtip promoter could work as expected in BL21. However, bacteria that have transformed this plasmid never shown any fluoresce, even though the sequence didn’t mutate. Later, we found that the sequence of qtip promoter contains more than one initiation codon. This may lead to the losing of efficiency of ECFP. So, we tried to delete sequence between eCFP and the last initiation codon and between eCFP and the penult one. Sad to say, we were not able to make this part work finally.
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<title>What？</title>
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<center><img src=" https://static.igem.wiki/teams/4645/wiki/quorum-sensing/cha-pqtip.png" style="width:25%; "></center>
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<center><b>Figure 1. The circuit to verify whether qtip promoter could work as expected
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 17:28, 11 October 2023


Pqitp

Promoter activated by VqmA in concert with dimethylpyrazin-2-ol (DPO).

Usage and Biology

We assembled Pqtip with BBa_K4645004then transfected into E. coli to sensing the density of bacteria and regulate the circuit.

Characterization

We inserted cCFP reporter gene behind qitp promoter to verify whether qtip promoter could work as expected in BL21. However, bacteria that have transformed this plasmid never shown any fluoresce, even though the sequence didn’t mutate. Later, we found that the sequence of qtip promoter contains more than one initiation codon. This may lead to the losing of efficiency of ECFP. So, we tried to delete sequence between eCFP and the last initiation codon and between eCFP and the penult one. Sad to say, we were not able to make this part work finally.

What？


Figure 1. The circuit to verify whether qtip promoter could work as expected

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]