Difference between revisions of "Part:BBa K4830027"

Revision as of 17:19, 11 October 2023

mCherry_C-terminus stop codon_Clover

The mCherry_C-terminus stop codon_Clover is protein coding sequence containing the mCherry fluorescent protein with a premature stop codon installed in the C-terminus before the Clover fluorescent protein. The presence of the premature stop codon prevents the expression of coding regions found downstream. This is particularly useful in assays such as the Traffic Light Reporter (TLR) assay.

Usage and Biology

The mCherry_C-terminus stop codon is utilized in the Traffic Light Reporter (TLR) assay, where a Clover fluorescent protein (commonly known as GFP) is found downstream. In this TLR assay, the Clover is not expressed due to the presence of the stop codon before the GFP. As such, the TLR assay is used to screen for DNA editing activity that swaps the stop codon into a functional amino acid, restoring the expression of Clover.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 352
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 352
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 352
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 352
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4830027 short</partinfo>
-The mCherry_C-terminus stop codon is protein coding sequence containing the mCherry fluorescent protein followed by a premature stop codon installed in the C-terminus. The presence of the premature stop codon prevents the expression of coding regions found downstream. This is particularly useful in assays such as the Traffic Light Reporter (TLR) assay.
+The mCherry_C-terminus stop codon_Clover is protein coding sequence containing the mCherry fluorescent protein with a premature stop codon installed in the C-terminus before the Clover fluorescent protein. The presence of the premature stop codon prevents the expression of coding regions found downstream. This is particularly useful in assays such as the Traffic Light Reporter (TLR) assay.
 ===Usage and Biology===
-The mCherry_C-terminus stop codon is utilized in the Traffic Light Reporter (TLR) assay, where a Clover fluorescent protein (commonly known as GFP) is found downstream. In this TLR assay, the Clover is not expressed due to the presence of the stop codon before the GFP.
+The mCherry_C-terminus stop codon is utilized in the Traffic Light Reporter (TLR) assay, where a Clover fluorescent protein (commonly known as GFP) is found downstream. In this TLR assay, the Clover is not expressed due to the presence of the stop codon before the GFP. As such, the TLR assay is used to screen for DNA editing activity that swaps the stop codon into a functional amino acid, restoring the expression of Clover.
 ===Sequence and Features===