Difference between revisions of "Part:BBa K4830027"

 
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The mCherry_C-terminus stop codon is protein coding sequence containing the mCherry fluorescent protein followed by a premature stop codon installed in the C-terminus. The presence of the premature stop codon prevents the expression of coding regions found downstream. This is particularly useful in assays such as the Traffic Light Reporter (TLR) assay.
 
The mCherry_C-terminus stop codon is protein coding sequence containing the mCherry fluorescent protein followed by a premature stop codon installed in the C-terminus. The presence of the premature stop codon prevents the expression of coding regions found downstream. This is particularly useful in assays such as the Traffic Light Reporter (TLR) assay.
  
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===Usage and Biology===
 
===Usage and Biology===
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The mCherry_C-terminus stop codon is utilized in the Traffic Light Reporter (TLR) assay, where a Clover fluorescent protein (commonly known as GFP) is found downstream. In this TLR assay, the Clover is not expressed due to the presence of the stop codon before the GFP.
  
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===Sequence and Features===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4830027 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4830027 SequenceAndFeatures</partinfo>
  

Revision as of 17:08, 11 October 2023


mCherry_C-terminus stop codon_Clover

The mCherry_C-terminus stop codon is protein coding sequence containing the mCherry fluorescent protein followed by a premature stop codon installed in the C-terminus. The presence of the premature stop codon prevents the expression of coding regions found downstream. This is particularly useful in assays such as the Traffic Light Reporter (TLR) assay.

Usage and Biology

The mCherry_C-terminus stop codon is utilized in the Traffic Light Reporter (TLR) assay, where a Clover fluorescent protein (commonly known as GFP) is found downstream. In this TLR assay, the Clover is not expressed due to the presence of the stop codon before the GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 352
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 352
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 352
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 352
  • 1000
    COMPATIBLE WITH RFC[1000]