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Revision as of 17:02, 11 October 2023
ZF21.16 minCMV promoter - Cre recombinase
Usage and Description
This composite part is a translational unit implemented in our project for conditional expression of Cre recombinase enzyme following stimulation of vimentin syn notch receptor. This enzyme controls the conditional expression of our booster genes (SDC4, STEAP3, NadB) (BBa_K4586027) by deleting (STOP) sequence flanked by two loxP sites upstream to the coding of our booster genes.
This figure illustrates the role of the Cre loxP system in regulation the expression of our booster genes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 673