Difference between revisions of "Part:BBa K187257"

Latest revision as of 21:56, 21 October 2009

trpB in pAB

trpB encodes the beta subunit of tryptophan synthase, and is present in the standard biobrick vector pSB1A3

This gene is present in plasmid pAB (part BBa_K187000), which is one of two universal plasmids for the BioBytes assembly method. For details of the BioBytes method, see RFC 47.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 10
Illegal PstI site found at 1210
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 1210
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 10
Illegal PstI site found at 1210
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 10
Illegal PstI site found at 1210
Illegal AgeI site found at 343
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K187257 short</partinfo>
-This primer is for a gene deemed to be essential for a minimal E.coli genome by Team Biobytes. This primer is designed to amplify only the open reading frame, and produce ends that can be digested by restriction enzymes for insertion into an XbaI/PstI digested plasmid. These restriction sites were designed to be complementary to the restrictions sites found in pAB and pBA (PstI and XbaI). Due to the use of restriction enzymes, it was important to check each gene sequence for those same restriction sites. Genes which contained at least one PstI site, used the NsiI enzyme to be cloned into our standard plasmids (it produces the same overhang as PstI). Similarly if NsiI and PstI were in the gene sequence, SbfI was used. If all three of these sites were present in a gene sequence, other enzymes which produce the same overhang could be used. Genes which contained XbaI could use AvrII, NheI, as well as a multitude of others.
+trpB encodes the beta subunit of tryptophan synthase, and is present in the standard biobrick vector pSB1A3
+This gene is present in plasmid pAB (part BBa_K187000), which is one of two universal plasmids for the BioBytes assembly method. For details of the BioBytes method, see RFC 47.
 <!-- Add more about the biology of this part here