Difference between revisions of "Part:BBa K4830025:Design"

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===Source===
 
===Source===
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The TLR-76 pegRNA design contains the spacer and RTTPBS sequence, which was adapted from other pegRNAs in the Prime Editor 2 paper cited in References. With the help of TWIST Bioscience we synthesized those and other necessary sequences, and further combined with 132777 backbone in our lab.
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===References===
 
===References===

Revision as of 16:39, 11 October 2023


TLR-76 pegRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence contains the spacer, gRNA scaffold and RTTPBS.

Source

The TLR-76 pegRNA design contains the spacer and RTTPBS sequence, which was adapted from other pegRNAs in the Prime Editor 2 paper cited in References. With the help of TWIST Bioscience we synthesized those and other necessary sequences, and further combined with 132777 backbone in our lab.

References