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===Functional Parameters===
 
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Revision as of 15:20, 11 October 2023


GFP with a protein degradation tag attached

A protein degradation tag (degron) is attached to the C-terminus of GFP to promote protein degradation.

Usage and Biology

While reviewing CRISPR-related materials, we discovered a particularly interesting evolutionary system- phage-assisted continuous evolution (PACE)[1,2], which can enable continuous directed evolution of gene-coding molecules that can be associated with protein production in E. coli. Then, we found a protein degradation tag-degron[3], that enables rapid hydrolysis of proteins with little functionality, which allows degron to be used in a number of protein co-expression screening systems.

Figure 1, Testing the protein degradation efficiency of degron. blank, LB medium. NT, E.coli without GFP expression plasmid, n=3.

Refrence

1. Esvelt, K. M., Carlson, J. C. & Liu, D. R. A system for the continuous directed evolution of biomolecules. Nature 472, 499-503 (2011). 2. Miller, S. M., Wang, T. & Liu, D. R. Phage-assisted continuous and non-continuous evolution. Nature Protocols 15, 4101-4127 (2020). 3. Karzai, A. W., Roche, E. D. & Sauer, R. T. The SsrA-SmpB system for protein tagging, directed degradation and ribosome rescue. Nat Struct Biol 7, 449-455 (2000).

Functional Parameters