Difference between revisions of "Part:BBa K4583053"

Revision as of 13:06, 11 October 2023

PHB production Regulation: PesaS-B0034-gltA-PesaRwt-B0034-PHBcab-PYU16-B0034-SRRz

PesaS-B0034-gltA-PesaRwt-B0034-PHBcab-PYU16-B0034-SRRz

Usage and Biology

This part is about PHB Production Regulation based on an three-layer dynamic regulation model. We successfully regulate the process of PHB production using this part.

1. Three-layer Dynamic Regulation Model

2. The first layer--Growth control

3. The second layer--Production control

4. The Third layer--Product-release control

Characterization

1. Protocols

2. The Characterization of PesaS and PesaRwt/PesaRc/PesaRp

3. The Characterization of PesaRwt/PesaRc/PesaRp and PYU3/PYU7/PYU16/PYU92

Summary of Results

Our part provides an example for future iGEM team

Limitations

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2740
Illegal BglII site found at 3565
Illegal BamHI site found at 1791
Illegal XhoI site found at 1511
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2046
Illegal NgoMIV site found at 2117
Illegal NgoMIV site found at 2717
Illegal NgoMIV site found at 3029
Illegal NgoMIV site found at 3308
Illegal NgoMIV site found at 3960
Illegal NgoMIV site found at 3982
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 5826
Illegal SapI.rc site found at 769

@@ Line 5: / Line 5: @@
 PesaS-B0034-gltA-PesaRwt-B0034-PHBcab-PYU16-B0034-SRRz
 ==Usage and Biology==
+This part is about PHB Production Regulation based on an three-layer dynamic regulation model. We successfully regulate the process of PHB production using this part.
 ===1. Three-layer Dynamic Regulation Model===
 ===2. The first layer--Growth control===
 ===3. The second layer--Production control===
 ===4. The Third layer--Product-release control===
 ==Characterization==
 ===1. Protocols===