Difference between revisions of "Part:BBa K4830018:Design"
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===Design Notes=== | ===Design Notes=== | ||
The original sequence of the protein was not altered in anyway. In order to use it for prime editing, this part has been fused to nickase Cas9 and combined with CMV promoter and backbone into plasmid. | The original sequence of the protein was not altered in anyway. In order to use it for prime editing, this part has been fused to nickase Cas9 and combined with CMV promoter and backbone into plasmid. | ||
| − | |||
===Source=== | ===Source=== | ||
| − | NCBI | + | NCBI ACCESSION: AAC82556 |
===References=== | ===References=== | ||
Revision as of 22:24, 11 October 2023
MMTV RT
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The original sequence of the protein was not altered in anyway. In order to use it for prime editing, this part has been fused to nickase Cas9 and combined with CMV promoter and backbone into plasmid.
Source
NCBI ACCESSION: AAC82556