Difference between revisions of "Part:BBa K4825003"

 
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===Usage and Biology===
 
===Usage and Biology===
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a heterogenous gene from Pinus taeda that can convert GPP into pinene. PS contains n-terminal transport peptides in the native host and is responsible for targeting translation products to plastid.
  
 
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Revision as of 16:56, 11 October 2023


PS

pinene synthase (PtPS) is a heterogenous gene from Pinus taeda that can convert GPP into pinene. PS contains n-terminal transport peptides in the native host and is responsible for targeting translation products to plastid, however this function is not required in yeast, and the N-terminal transport peptide might cause erring location of the enzyme, decrease in solubility or wrongful folding of the enzyme and affect the efficiency in yeasts, so the N-terminal of PtPS is truncated to form tPtPS

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 223
    Illegal BamHI site found at 1060
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 695