Difference between revisions of "Part:BBa K4645012:Design"

Latest revision as of 14:21, 12 October 2023

The RstAB system is a bacterial two-component regulatory system consisting of the membrane sensor

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 324
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1501

Design Notes

The RstA/RstB system is a bacterial two-component regulatory system consisting of the membrane sensor, RstB and its cognate response regulator RstA. The RstA consists of an N-terminal receiver domain (RD, residues 1–119) and a C-terminal DNA-binding domain (DBD, residues 130–236). Phosphorylation of RstA induces dimerization, which allows two RstA DBDs to bind to a tandem repeat, called the RstA box, and regulate the expression of downstream genes [1]. In the sequence of the acid-sensitive promoter asr, there is a RstA box [2].

Source

The team obtained the RstAB fragment from the E. coli MG1655 genome by PCR using a high-fidelity DNA polymerase.

Reference

[1]Li YC, Chang CK, Chang CF, Cheng YH, Fang PJ, Yu T, Chen SC, Li YC, Hsiao CD, Huang TH.Structural dynamics of the two-component response regulator RstA in recognition of promoter DNA element. Nucleic Acids Res. 2014 Jul;42(13):8777-88.

[2]Ogasawara H, Hasegawa A, Kanda E, Miki T, Yamamoto K, Ishihama A. Genomic SELEX search for target promoters under the control of the PhoQP-RstBA signal relay cascade. J Bacteriol. 2007 Jul;189(13):4791-9.

@@ Line 7: / Line 7: @@
 ===Design Notes===
-The RstA/RstB system is a bacterial two-component regulatory system consisting of the membrane sensor, RstB and its cognate response regulator  RstA.The RstA consists of an N-terminal receiver domain (RD, residues 1–119) and a C-terminal DNA-binding domain (DBD, residues 130–236). Phosphorylation of RstA induces dimerization, which allows two RstA DBDs to bind to a tandem repeat, called the RstA box, and regulate the expression of downstream genes[1]. In the sequence of the acid-sensitive promoter P asr, there is a RstA box[2].
+The RstA/RstB system is a bacterial two-component regulatory system consisting of the membrane sensor, RstB and its cognate response regulator RstA. The RstA consists of an N-terminal receiver domain (RD, residues 1–119) and a C-terminal DNA-binding domain (DBD, residues 130–236). Phosphorylation of RstA induces dimerization, which allows two RstA DBDs to bind to a tandem repeat, called the RstA box, and regulate the expression of downstream genes [1]. In the sequence of the acid-sensitive promoter asr, there is a RstA box [2].
 ===Source===
-The team obtained the RstAB fragment from the E. coli MG1655 genome by PCR using a high-fidelity DNA polymerase.
+The team obtained the RstAB fragment from the <i>E. coli</i> MG1655 genome by PCR using a high-fidelity DNA polymerase.
 ===Reference===
 <p>[1]Li YC, Chang CK, Chang CF, Cheng YH, Fang PJ, Yu T, Chen SC, Li YC, Hsiao CD, Huang TH.Structural dynamics of the two-component response regulator RstA in recognition of promoter DNA element. Nucleic Acids Res. 2014 Jul;42(13):8777-88.</p>
 <p>[2]Ogasawara H, Hasegawa A, Kanda E, Miki T, Yamamoto K, Ishihama A. Genomic SELEX search for target promoters under the control of the PhoQP-RstBA signal relay cascade. J Bacteriol. 2007 Jul;189(13):4791-9.</p>