Difference between revisions of "Part:BBa K4830017"

 
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<partinfo>BBa_K4830017 short</partinfo>
 
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The Lactococcus lactis group II intron Ll.ltrB is similar to mobile yeast mtDNA group II introns, which encode reverse transcriptase, RNA maturase, and DNA endonuclease activities for site-specific DNA insertion.
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Ll.ltrA reverse transcriptase is one of proteins found in The Lactococcus lactis Ll.LtrB intron, that binds the intron RNA to promote RNA splicing and intron mobility.  
  
 
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===Usage and Biology===
 
===Usage and Biology===
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Ll.ltrA was one of the Reverse Transcriptases we attempted to use as alternative RT for Prime editing.
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===Characterization===
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To evaluate the performance of various Ll.ltrA, we cotransfected the RT with pegRNA and ngRNA targeting the RNF and HEK3 genomic loci on HEK293T cells and estimated the editing efficiency using sanger sequencing. Another evaluation test was performed on TLR reporter system at 24hr and 72hr transfection time.The results are plotted as bar graph shown below. Comparison was done against the wild-type PE2 and PE2 with truncated RNAse H region (PE-497).
  
 
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Revision as of 12:03, 11 October 2023


Ll.ltrA RT

Ll.ltrA reverse transcriptase is one of proteins found in The Lactococcus lactis Ll.LtrB intron, that binds the intron RNA to promote RNA splicing and intron mobility.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]