Difference between revisions of "Part:BBa K4960003"
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<partinfo>BBa_K4960003 short</partinfo> | <partinfo>BBa_K4960003 short</partinfo> | ||
| − | === | + | ===Profile=== |
| − | + | Name: Pvc3<br> | |
| − | Pvc3 is a part of the sheath. | + | Base Pairs: 1323 bp<br> |
| − | + | Origin: Photorhabdus<br> | |
| + | Properties: Pvc3 is a part of the sheath.<br> | ||
<!-- Add more about the biology of this part here--> | <!-- Add more about the biology of this part here--> | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices and present in both bacteria and archaea.[1]In structure, PVCs are macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2] | + | Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices and present in both bacteria and archaea.[1]In structure, PVCs are macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure 1) |
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| − | <img src="https://static.igem.wiki/teams/4960/wiki/basic-part/ | + | <img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc1-16.jpg" class="figure-img img-fluid rounded" height="150px"> |
</figure> | </figure> | ||
</html> | </html> | ||
| − | + | Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).<br> | |
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Pvc3 consists of the sheath with Pvc2 and Pvc4. | Pvc3 consists of the sheath with Pvc2 and Pvc4. | ||
Pvc3 contains an extra protrusion domain, which is used for tailfiber docking, is in a comparable position of domain III in the T6SS sheath. | Pvc3 contains an extra protrusion domain, which is used for tailfiber docking, is in a comparable position of domain III in the T6SS sheath. | ||
A part of sheath, comprises alternating Pvc2 and Pvc3 subunits, which both interact with Pvc1. At the end of assembly, Pvc3 starts to assemble along the inner tube for the maturation of PVC particles with Pvc2. | A part of sheath, comprises alternating Pvc2 and Pvc3 subunits, which both interact with Pvc1. At the end of assembly, Pvc3 starts to assemble along the inner tube for the maturation of PVC particles with Pvc2. | ||
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===Sequence and Features=== | ===Sequence and Features=== | ||
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<partinfo>BBa_K4960003 parameters</partinfo> | <partinfo>BBa_K4960003 parameters</partinfo> | ||
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| − | === | + | ===References=== |
| − | [1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;<br> | + | [1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29. <br> |
| − | [2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4. | + | [2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21. <br> |
Revision as of 12:08, 11 October 2023
pvc3
Profile
Name: Pvc3
Base Pairs: 1323 bp
Origin: Photorhabdus
Properties: Pvc3 is a part of the sheath.
Usage and Biology
Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices and present in both bacteria and archaea.[1]In structure, PVCs are macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure 1)
Pvc3 consists of the sheath with Pvc2 and Pvc4.
Pvc3 contains an extra protrusion domain, which is used for tailfiber docking, is in a comparable position of domain III in the T6SS sheath.
A part of sheath, comprises alternating Pvc2 and Pvc3 subunits, which both interact with Pvc1. At the end of assembly, Pvc3 starts to assemble along the inner tube for the maturation of PVC particles with Pvc2.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 452
Illegal XhoI site found at 736 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29.
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21.