Difference between revisions of "Part:BBa K4604005"
Line 4: Line 4:
  
 
<html>
 
<html>
−
Gene for BluB enzyme, catalyzes the reaction of FMNH2 to DMB used in the AdoCbl synthesis pathway in E. coli. Used in BBa_K4604015, BBa_K4604016,</html>
+
Gene for BluB enzyme, catalyzes the reaction of FMNH2 to DMB used in the AdoCbl synthesis pathway in <i>E. coli</i>. Ultimately leads to AdoCbl (B12) synthesis. Also used in <a href="https://parts.igem.org/Part:BBa_K4604015">BBa_K4604015</a>, <a href="https://parts.igem.org/Part:BBa_K4604016">BBa_K4604016</a>, <a href="https://parts.igem.org/Part:BBa_K4604021">BBa_K4604021</a>, <a href="https://parts.igem.org/Part:BBa_K4604022">BBa_K4604022</a> and <a href="https://parts.igem.org/Part:BBa_K4604023">BBa_K4604023</a>. A more detailed characterization is uploaded on these respective registry pages. </html>
  
  
Line 15: Line 15:
  
  
−
===Usage and Biology===
+
 
  
 
<!-- -->
 
<!-- -->

Revision as of 09:01, 11 October 2023


BluB

Gene for BluB enzyme, catalyzes the reaction of FMNH2 to DMB used in the AdoCbl synthesis pathway in E. coli. Ultimately leads to AdoCbl (B12) synthesis. Also used in BBa_K4604015, BBa_K4604016, BBa_K4604021, BBa_K4604022 and BBa_K4604023. A more detailed characterization is uploaded on these respective registry pages.


Figure 1: Vitamin B12 production from precursors in E. coli. The BluB transforms the reduced form of flavin mononucleotide (FMNH2) to form 5,6-dimethylbenzimidazole (DMB) which then joins exogenously-provided cobinamide to create vitamin B12.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]