Difference between revisions of "Part:BBa K4785004:Experience"

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===Applications of BBa_K4785004===
 
===Applications of BBa_K4785004===
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Protein function verification, Cytotoxicity Test and Inflammation Verification for PslG can be seen in the Part:BBa_K4785005
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1.Protein function verification
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In the inhibition of biofilm growth experiment, we can observe that HMGB1_FL box shows the strongest ability to inhibit the biofilm growth among four HMGB1 modified proteins.
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        <img src="https://static.igem.wiki/teams/4785/wiki/pslg-inhibition.png" width="500" height="auto">
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Figure 1: Time course of PslG effect on biofilm growth. The biofilm biomass was measured by crystal violet assay.
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In the biofilm disassembly experiment, the HMGB1_FL shows the strongest ability to disassemble the biofilm among four HMGB1 modified proteins.
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        <img src="https://static.igem.wiki/teams/4785/wiki/pslg-disassembly.png" width="500" height="auto">
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Figure 2: Time course of PslGeffect on biofilm disassembly. The biofilm biomass was measured by crystal violet assay.
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According to those results, we decided to choose PslG and HMGB1_FL as our functional proteins.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 08:20, 12 October 2023


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4785004

1.Protein function verification

In the inhibition of biofilm growth experiment, we can observe that HMGB1_FL box shows the strongest ability to inhibit the biofilm growth among four HMGB1 modified proteins.

Figure 1: Time course of PslG effect on biofilm growth. The biofilm biomass was measured by crystal violet assay. In the biofilm disassembly experiment, the HMGB1_FL shows the strongest ability to disassemble the biofilm among four HMGB1 modified proteins.

Figure 2: Time course of PslGeffect on biofilm disassembly. The biofilm biomass was measured by crystal violet assay. According to those results, we decided to choose PslG and HMGB1_FL as our functional proteins.

User Reviews

UNIQ929a8c091e0a02d2-partinfo-00000002-QINU UNIQ929a8c091e0a02d2-partinfo-00000003-QINU