Difference between revisions of "Part:BBa K4800055"
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− | <partinfo> | + | <partinfo>BBa_K4800013 short</partinfo> |
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+ | The plasmid of pRSFDuet-sfp-MmCAR-YahK were constructed to obtain the functional modules for 1,5-pentanediol.We co-transformed the plasmid of pTrc99a-davB-davA-GabT and pRSFDuet-sfp-MmCAR-YahK into a lysine producing strain <i>E. coli</i> NT1003 to produce 1,5-PDO from glucose. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | <html lang="en"> | ||
− | < | + | <head> |
− | === | + | <meta charset="UTF-8"> |
− | <partinfo> | + | <meta http-equiv="X-UA-Compatible" content="IE=edge"> |
− | < | + | <meta name="viewport" content="width=device-width, initial-scale=1.0"> |
+ | <title>Document</title> | ||
+ | </head> | ||
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+ | <body> | ||
+ | <p style="font-size: 150%; font-weight: bold;">Build: | ||
+ | <div align="center"> | ||
+ | <p><centre><img src="https://static.igem.wiki/teams/4800/wiki/parts/bba-k4800013-1.png" width="50%" height="30%"></centre></p> | ||
+ | </div> | ||
+ | <div align="center"> | ||
+ | <strong>Figure 1. The schematic of plasmid pRSFDuet-sfp-MmCAR-YahK.</strong> | ||
+ | </div> | ||
+ | <p>The genes of 1,5-PDO synthesis module including MmCAR and YahK were subcloned into the plasmid pACYCDuet together under the control of trc promoter to yield plasmid pACYCDuet-sfp-MmCAR-YahK. In detail, based on the sequence of an existing part of <partinfo>BBa_K1655000</partinfo> , it was codon-optimized and synthesized to obtain the fragment of sfp-MmCAR, which was then ligated into the pACYCDuet plasmid under the control of trc promoter. The Ptrc-YahK fragment was amplified with plasmid pTrc99a-YahK as the template, and In-Fusion cloning was then used to ligate into the above vectors to yield plasmid pACYCDuet-sfp-MmCAR-YahK. The plasmid were transformed into <i>E. coli</i> Trans-T1, and corrected colonies were verified by PCR, and sequencing. | ||
+ | </p > | ||
+ | <p style="font-size: 150%; font-weight: bold;">Test: | ||
+ | <div align="center"> | ||
+ | <p><centre><img src="https://static.igem.wiki/teams/4800/wiki/parts/bba-k4800013-1.png" width="50%" height="30%"></centre></p> | ||
+ | </div> | ||
+ | <div align="center"> | ||
+ | <strong>Figure 2. |
Revision as of 10:07, 12 October 2023
_NOTOC__ YcjQ sgRNA
The plasmid of pRSFDuet-sfp-MmCAR-YahK were constructed to obtain the functional modules for 1,5-pentanediol.We co-transformed the plasmid of pTrc99a-davB-davA-GabT and pRSFDuet-sfp-MmCAR-YahK into a lysine producing strain E. coli NT1003 to produce 1,5-PDO from glucose.
Build:
The genes of 1,5-PDO synthesis module including MmCAR and YahK were subcloned into the plasmid pACYCDuet together under the control of trc promoter to yield plasmid pACYCDuet-sfp-MmCAR-YahK. In detail, based on the sequence of an existing part of
Test: