Difference between revisions of "Part:BBa K4604033"
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | Riboswitches occur naturally as regulators of gene expression. Riboswitches are encoded as DNA sequences which exert their regulatory effect upon transcription by folding into a complex 3D structure. Generally, a riboswitch can fold in such a way that the ribosome binding site (RBS) is available or unavailable for the ribosome. Upon binding to the target compound, like AdoCbl, a configurational change in the riboswitch changes the availability of the RBS | + | Riboswitches occur naturally as regulators of gene expression. Riboswitches are encoded as DNA sequences which exert their regulatory effect upon transcription by folding into a complex 3D structure. Generally, a riboswitch can fold in such a way that the ribosome binding site (RBS) is available or unavailable for the ribosome. Upon binding to the target compound, like AdoCbl, a configurational change in the riboswitch changes the availability of the RBS. |
Revision as of 23:44, 10 October 2023
SY AdoCbl riboswitch
This Biobrick is a riboswitch for AdoCbl, a bioavailable form of B12. Upon binding to AdoCbl, a conformational change happens within the riboswitch, negatively regulating the gene downstream of it. To verify the performance of the riboswitch we created several sensors with a fluorescent readout: BBa_K4604026, BBa_K4604027, BBa_K4604028 and BBa_K4604029.
Usage and Biology
Riboswitches occur naturally as regulators of gene expression. Riboswitches are encoded as DNA sequences which exert their regulatory effect upon transcription by folding into a complex 3D structure. Generally, a riboswitch can fold in such a way that the ribosome binding site (RBS) is available or unavailable for the ribosome. Upon binding to the target compound, like AdoCbl, a configurational change in the riboswitch changes the availability of the RBS.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 167
- 1000COMPATIBLE WITH RFC[1000]