Difference between revisions of "Part:BBa K4960027"

Revision as of 20:52, 10 October 2023 (view source) Palpitate (Talk | contribs) ← Older edit		Revision as of 18:44, 11 October 2023 (view source) CirceTerry (Talk | contribs) Newer edit →
Line 5:		Line 5:
	===Profile===		===Profile===
−	Name:pvc13_NTD-3*GGGSG-CKGGRAKDC-3*GGGSG-pvc13_CTD<br>	+	Name:pvc13_NTD-3*GGSGG-CKGGRAKDC-3*GGSGG-pvc13_CTD<br>
	Base Pairs: 1422bp<br>		Base Pairs: 1422bp<br>
	Origin: Synthetic<br>		Origin: Synthetic<br>
Line 15:		Line 15:
	<figure class="figure">		<figure class="figure">
−	<p><img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc25-26-27-28-29/1.png" alt="" width="800" height="1000" /></p>	+	<p><img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc25-26-27-28-29/1.png" alt="" width="400" height="500" /></p>
	</figure>		</figure>
Line 24:		Line 24:
	<figure class="figure">		<figure class="figure">
−	<p><img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc25-26-27-28-29/4.png" alt="" width="800" height="1000" /></p>	+	<p><img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc25-26-27-28-29/4.png" alt="" width="300" height="375" /></p>
	</figure>		</figure>
Line 33:		Line 33:
	<figure class="figure">		<figure class="figure">
−	<p><img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc25-26-27-28-29/2.png" alt="" width="800" height="600" /></p>	+	<p><img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc25-26-27-28-29/2.png" alt="" width="400" height="300" /></p>
	</figure>		</figure>

---

Revision as of 18:44, 11 October 2023

pvc13 NTD-3*GGSGG-CKGGRAKDC-3*GGSGG-pvc13 CTD

A flexible linker which can influence the structure of tail fiber connected to the CKGGRAKDC.

Profile

Name:pvc13_NTD-3*GGSGG-CKGGRAKDC-3*GGSGG-pvc13_CTD
Base Pairs: 1422bp
Origin: Synthetic
Properties: A flexible linker which can influence the structure of tail fiber connected to the CKGGRAKDC.

Usage and Biology

As an indispensable component of recombinant fusion proteins, linkers have shown increasing importance in the construction of bioactive fusion proteins.[1] (GGGGS)n is a commonly used flexible linker.[1] Here, we use (GGGGS)3 to influence the structure of tail fiber connected to the CKGGRAKDC.

Figure 1.Schematic diagram of the influence of different Linkers on the structure of tail fibers

Figure 2.PVC illustration

Figure 3.Horizontal cutaway view of the fiber region of the syringe.

Special Design

Extend the flexible linker on both sides of the CKGGRAKDC to make the CKGGRAKDC more visibly exposed, which in turn facilitates more precise targeting of adipocytes.

Sequence and Features

References

[1] Chen, X., Zaro, J. L., & Shen, W. C. (2013). Fusion protein linkers: property, design and functionality. Advanced drug delivery reviews, 65(10), 1357–1369.