Difference between revisions of "Part:BBa K4613012"

Revision as of 09:39, 11 October 2023

C3

This part codes for hydrophilic elastin-like polypeptides(ELPs) fused with triple SpyCatcher sequences, through polymerization by covalent bonding between SpyTag and SpyCatcher, forming polymeric scaffolds. If you want to learn about the detailed introduction of BSS, you can click the link below. https://parts.igem.org/Part:BBa_K4613010

Fig.1 Verification of the fabrication between T3-YFP and C3. Lane1: T3-YFP. Lane2: C3. M: Marker. Lane3: T3-YFP and C3(4℃, 8h). Lane4: T3-YFP and C3(4℃, 3h). Lane5: T3-YFP and C3(4℃, 1h). Lane6: T3-YFP and C3(25℃, 8h). Lane7: T3-YFP and C3(25℃, 3h). Lane8: T3-YFP and C3(25℃, 1h). Lane9: T3-YFP and C3(37℃，8h). Lane10: T3-YFP and C3(37℃, 3h). Lane11: T3-YFP and C3(37℃, 1h).

Reference

1. Dai Z, Yang X, Wu F, et al.Living fabrication of functional semi-interpenetrating polymeric materials[J].Nat Commun,2021, 12 (1): 3422.
2. Zakeri B, Fierer J O, Celik E, et al.Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin[J].Proc Natl Acad Sci U S A,2012, 109 (12): E690-7.
3. Reddington S C, Howarth M.Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher[J].Curr Opin Chem Biol,2015, 29: 94-9.

Sequence and Features