Difference between revisions of "Part:BBa K4830015"

 
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<partinfo>BBa_K4830015 short</partinfo>
 
<partinfo>BBa_K4830015 short</partinfo>
  
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Prototype Foamy virus Reverse transcriptase.
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Prototype Foamy virus Reverse transcriptase.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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PFV RT retrieved from chimpanzee was human codon optimized and fused to nickase Cas9 to form prime editor.
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===Characterization===
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It was tested on endogeneous sites as well TLR reporter using HEK293T cells to evaluate the alternative RT performance in terms of its ability to introduce different edits on different target sites.
  
 
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Revision as of 21:06, 10 October 2023


PFV RT

Prototype Foamy virus Reverse transcriptase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]