Difference between revisions of "Part:BBa K4830014:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Codon optimization | + | Codon optimization to avoid illegal Restriction sites. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | |||
pCMV-PE2 (H840A) | pCMV-PE2 (H840A) | ||
Addgene Plasmid #132775 | Addgene Plasmid #132775 | ||
===References=== | ===References=== | ||
| + | Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature. 2019 Oct 21;576. | ||
Revision as of 20:33, 10 October 2023
nickase Cas9
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimization to avoid illegal Restriction sites.
Source
pCMV-PE2 (H840A) Addgene Plasmid #132775
References
Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature. 2019 Oct 21;576.