Difference between revisions of "Part:BBa K4960012"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K4960012 short</partinfo>
 
<partinfo>BBa_K4960012 short</partinfo>
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===Properties===
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===Profile===
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Name: pvc12<br>
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PVC12 is a part of PVC Baseplate.
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Base Pairs: 2853 bp<br>
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Origin: Photorhabdus<br>
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Properties: Pvc12 is a part of pvc baseplate.<br>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
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In the assembled wedges, six copies of Pvc11-Pvc12 heterodimers form a hexagonal ring through the dimerization between the domain V of Pvc11 and the domain III of Pvc12 from a neighboring heterodimer.
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Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1)<br>
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Two sides of hexameric rings are found opposite surface charges, PVC12 included.  
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<html>
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The evolutionary relationship between Pvc12 and known wedge proteins is not apparent because of the low sequence conservation.
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In the six helices of the Pvc11Pvc12 dimer, four of which in Pvc12 are equivalent to the ones seen in the gp6-gp7 dimer.
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<figure class="figure">
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<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc1-16.jpg" class="figure-img img-fluid rounded"  height="150px">
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</figure>
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</html>
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Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).<br>
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<br>
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In the assembled wedges, six copies of Pvc11-Pvc12 heterodimers form a hexagonal ring through the dimerization between the domain V of Pvc11 and the domain III of Pvc12 from a neighboring heterodimer.<br>
 +
Two sides of hexameric rings are found opposite surface charges, PVC12 included. <br>
 +
The evolutionary relationship between Pvc12 and known wedge proteins is not apparent because of the low sequence conservation.<br>
 +
In the six helices of the Pvc11Pvc12 dimer, four of which in Pvc12 are equivalent to the ones seen in the gp6-gp7 dimer.<br>
 
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===Sequence and Features===
 
===Sequence and Features===
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<!-- Uncomment this to enable Functional Parameter display -->
 
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===References===
 
===References===
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[1] J. Kreitz et al., “Programmable protein delivery with a bacterial contractile injection system,” Nature, vol. 616, no. 7956, pp. 357–364, Apr. 2023, doi: 10.1038/s41586-023-05870-7.<br>
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[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29. <br>
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[2] F. Jiang et al., “Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System,” Cell, vol. 177, no. 2, pp. 370-383.e15, Apr. 2019, doi: 10.1016/j.cell.2019.02.020.
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[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21. <br>

Revision as of 12:47, 11 October 2023


pvc12

Profile

Name: pvc12
Base Pairs: 2853 bp
Origin: Photorhabdus
Properties: Pvc12 is a part of pvc baseplate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 327
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 855
    Illegal AgeI site found at 639
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29.
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21.