Difference between revisions of "Part:BBa K4960012"

 
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<partinfo>BBa_K4960012 short</partinfo>
 
<partinfo>BBa_K4960012 short</partinfo>
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===Properties===
  
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PVC12 is a part of PVC Baseplate.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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In the assembled wedges, six copies of Pvc11-Pvc12 heterodimers form a hexagonal ring through the dimerization between the domain V of Pvc11 and the domain III of Pvc12 from a neighboring heterodimer.
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Two sides of hexameric rings are found opposite surface charges, PVC12 included.
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The evolutionary relationship between Pvc12 and known wedge proteins is not apparent because of the low sequence conservation.
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In the six helices of the Pvc11Pvc12 dimer, four of which in Pvc12 are equivalent to the ones seen in the gp6-gp7 dimer.
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4960012 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4960012 SequenceAndFeatures</partinfo>
  
  
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===Functional Parameters===
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===References===
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<partinfo>BBa_K4960012 parameters</partinfo>
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[1] J. Kreitz et al., “Programmable protein delivery with a bacterial contractile injection system,” Nature, vol. 616, no. 7956, pp. 357–364, Apr. 2023, doi: 10.1038/s41586-023-05870-7.<br>
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[2] F. Jiang et al., “Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System,” Cell, vol. 177, no. 2, pp. 370-383.e15, Apr. 2019, doi: 10.1016/j.cell.2019.02.020.

Revision as of 20:02, 10 October 2023


pvc12

Properties

PVC12 is a part of PVC Baseplate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 327
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 855
    Illegal AgeI site found at 639
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] J. Kreitz et al., “Programmable protein delivery with a bacterial contractile injection system,” Nature, vol. 616, no. 7956, pp. 357–364, Apr. 2023, doi: 10.1038/s41586-023-05870-7.
[2] F. Jiang et al., “Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System,” Cell, vol. 177, no. 2, pp. 370-383.e15, Apr. 2019, doi: 10.1016/j.cell.2019.02.020.