Difference between revisions of "Part:BBa K4960005"

Revision as of 12:16, 11 October 2023

pvc5

Profile

Name: pvc5
Base Pairs:459 bp
Origin:Photorhabdus
Properties: guide the synthesis of the PVC tube initiator

Usage and Biology

Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins.[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure 1)

Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).

The PVC tube linked with payloads consists of three structurally similar tube proteins, such that a complete syringe in a typical length contains 24 stacked hexameric rings of tube proteins, including 2 layers of tube initiators and 22 layers of main tube proteins. Among all related genes,pvc5 produces pvc5 hexameric rings which stacks onto the Pvc7 ring to form the first two initiator rings in the baseplate end of the PVC tube through opposite surface charges and electrostatic attraction.Furthermore, pvc5 is encircled to form the first layer of the sheath which aims to prepare for the contraction in the delivery process.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29.
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4960005 short</partinfo>
-===Properties===
+===Profile===
+Name: pvc5<br>
-guide the synthesis of the PVC tube initiator
+Base Pairs:459 bp<br>
+Origin:Photorhabdus<br>
+Properties: guide the synthesis of the PVC tube initiator<br>
 <!-- Add more about the biology of this part here-->
 ===Usage and Biology===
-Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Fig.a,Fig.b)<br>
+Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins.[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure 1)<br>
 <html>
 <figure class="figure">
-<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc2-3-4-5-6-7.png" class="figure-img img-fluid rounded"  height="200px">
+<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc1-16.jpg" class="figure-img img-fluid rounded"  height="150px">
 </figure>
 </html>
-a. PVC illustrations
+Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).<br>
-b.Payload illustrations
+<br>
+The PVC tube linked with payloads consists of three structurally similar tube proteins, such that a complete syringe in a typical length contains 24 stacked hexameric rings of tube proteins, including 2 layers of tube initiators and 22 layers of main tube proteins. Among all related genes,pvc5 produces pvc5 hexameric rings which stacks onto the Pvc7 ring to form the first two initiator rings in the baseplate end of the PVC tube through opposite surface charges and electrostatic attraction.Furthermore, pvc5 is encircled to form the first layer of the sheath which aims to prepare for the contraction in the delivery process.
-The PVC tube linked with payloads consists of three structurally similar tube proteins, such that a complete syringe in a typical length contains 24 stacked hexameric rings of tube proteins, including 2 layers of tube initiators and 22 layers of main tube proteins. Among all related genes,pvc5 produces pvc5 hexameric rings which stacks onto the Pvc7 ring to form the first two initiator rings(Fig.c) in the baseplate end of the PVC tube through opposite surface charges and electrostatic attraction.Furthermore, pvc5 is encircled to form the first layer of the sheath which aims to prepare for the contraction in the delivery process.(Fig.d)
-<html>
-<figure class="figure">
-<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc-1-5-7.png"class="figure-img img-fluid rounded"  height="200px">
-</figure>
-</html>
-c.the direction of pvc5<br>
-d.The cross-sectional view of pvc5 with other pvcs
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+===Sequence and Features===
 <partinfo>BBa_K4960005 SequenceAndFeatures</partinfo>
@@ Line 43: / Line 34: @@
 <!-- -->
 ===References===
-[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;
+[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29. <br>
-[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;
+[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21. <br>