Difference between revisions of "Part:BBa K4960006"

Revision as of 12:19, 11 October 2023

pvc6

Profile

Name: pvc6
Base Pairs: 183 bp
Origin: Photorhabdus
Properties:guide the synthesis of the PVC

Usage and Biology

Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure 1)

Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).

Among all related genes,Pvc6 is the shortest ORF in the PVC gene clusters.Based on the present research,pvc6 possibly functions together with Pvc14 in determining the optimal length of PVC particles.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29.
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21.

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K4960006 short</partinfo>
-===Properties===
+===Profile===
+Name: pvc6<br>
-guide the synthesis of the PVC.
+Base Pairs: 183 bp<br>
+Origin: Photorhabdus<br>
+Properties:guide the synthesis of the PVC<br>
 <!-- Add more about the biology of this part here-->
 ===Usage and Biology===
-Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Fig.a,Fig.b)<br>
+Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure 1)<br>
 <html>
 <figure class="figure">
-<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc2-3-4-5-6-7.png" class="figure-img img-fluid rounded"  height="200px">
+<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc1-16.jpg" class="figure-img img-fluid rounded"  height="150px">
 </figure>
 </html>
-a. PVC illustrations<br>
+Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).<br>
-b.Payload illustrations<br>
+<br>
 Among all related genes,Pvc6 is the shortest ORF in the PVC gene clusters.Based on the present research,pvc6 possibly functions together with Pvc14 in determining the optimal length of PVC particles.
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+===Sequence and Features===
 <partinfo>BBa_K4960006 SequenceAndFeatures</partinfo>
@@ Line 32: / Line 34: @@
 <!-- -->
 ===References===
-[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;<br>
+[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29. <br>
-[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;
+[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21. <br>