Difference between revisions of "Part:BBa K4724075:Design"
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===Design Notes=== | ===Design Notes=== |
Latest revision as of 19:23, 10 October 2023
NusA-LSPET
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1368
Illegal XhoI site found at 2344 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1600
Illegal AgeI site found at 1687 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The DNA of LSPETase and NusA were both inserted into a plasmid, resulting in the construction of a recombinant plasmid. Subsequently, transcription and translation were carried out, leading to the fusion of NusA at the N-terminus of LSPETase.
Source
Laboratory design in our research group.