Difference between revisions of "Part:BBa K4634018"

 
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Here we hope to optimize PL promoter, enabling a higher expression level at 42 °C. The following content mainly describes the pBV220_PL2 which shows prominent improvement during our experiment. Considering the completeness of the project, the nuclear acid sequence as well as corresponding experimental data of other promoters are also listed below.
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<h2>Characterization</h2>
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<h3>Introduction</h3>
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PL and PR promoter, which are ubiquitously employed in bacterial expression systems, are typical promoters regulated by temperature and . In pBV220, they are located tandemly. The repressor, Tcl857, is a temperature sensitive mutant of phage λ. Under normal condition, Tcl857 act as dimers that bind to the OR1, OR2 and OR3 operator site, hindering the occurrence of transcription. When the temperature rises to about 42 °C, the repressor is thus denatured and the transcription happens.[1]
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<h3>Usage and biology</h3>
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Compared to PL wild type, the Temperature-sensitive promoter X shows a higher promoter activity after heat treatment.
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Revision as of 05:23, 12 October 2023


Temperature-sensitive Promoter: pBV220_PL-2, modified from PL promoter.

Here we hope to optimize PL promoter, enabling a higher expression level at 42 °C. The following content mainly describes the pBV220_PL2 which shows prominent improvement during our experiment. Considering the completeness of the project, the nuclear acid sequence as well as corresponding experimental data of other promoters are also listed below.

Characterization

Introduction

PL and PR promoter, which are ubiquitously employed in bacterial expression systems, are typical promoters regulated by temperature and . In pBV220, they are located tandemly. The repressor, Tcl857, is a temperature sensitive mutant of phage λ. Under normal condition, Tcl857 act as dimers that bind to the OR1, OR2 and OR3 operator site, hindering the occurrence of transcription. When the temperature rises to about 42 °C, the repressor is thus denatured and the transcription happens.[1]

Usage and biology

Compared to PL wild type, the Temperature-sensitive promoter X shows a higher promoter activity after heat treatment. Sequence and Features BBa_K4634018 SequenceAndFeatures