Difference between revisions of "Part:BBa K4800041"
 
Line 3: Line 3:
 
<partinfo>BBa_K4800041 short</partinfo>
 
<partinfo>BBa_K4800041 short</partinfo>
  
−
Putrescine-2-oxoglutarate transaminase from <i>Escherichia coli</i> is a pyridoxal 5'-phosphate protein.It catalyzes the formation of glutamic acid from glutamine
+
We linearized the pTrc99a vector using restriction endonuclease and connected the target gene PatA to it through homologous recombination to obtain the recombinant plasmid pTrc99a-PatA.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 08:41, 11 October 2023


pTrc99a-PatA

We linearized the pTrc99a vector using restriction endonuclease and connected the target gene PatA to it through homologous recombination to obtain the recombinant plasmid pTrc99a-PatA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4036
    Illegal PstI site found at 4048
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 4048
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4030
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4036
    Illegal PstI site found at 4048
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4036
    Illegal PstI site found at 4048
    Illegal NgoMIV site found at 2804
    Illegal AgeI site found at 3311
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4232
    Illegal BsaI site found at 5300
    Illegal SapI.rc site found at 767