Difference between revisions of "Part:BBa K4830007:Design"

 
 
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===Design Notes===
 
===Design Notes===
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The design of HEK3 pegRNA includes the U6 promoter, spacer, guide RNA scaffold, rttpbs and polyA tail.
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The design of HEK3 ngRNA includes the U6 promoter, spacer, guide RNA scaffold, and polyA tail.
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+
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===Source===
 
===Source===
  
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The original HEK3 pegRNA design containing spacer and rttpbs sequence was reported in Prime Editor 2 paper cited in References. With the help of TWIST Bioscience we synthesized those and other necessary sequences, and further combined with 65777 backbone in our lab.
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The original HEK3 ngRNA design containing spacer sequence was reported in Prime Editor 2 paper cited in References. With the help of TWIST Bioscience we synthesized that sequence and further pieced together with 65777 backbone in our lab.
  
 
===References===
 
===References===
 +
Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature. 2019 Oct 21;576.

Latest revision as of 17:07, 10 October 2023


HEK3 ngRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design of HEK3 ngRNA includes the U6 promoter, spacer, guide RNA scaffold, and polyA tail.

Source

The original HEK3 ngRNA design containing spacer sequence was reported in Prime Editor 2 paper cited in References. With the help of TWIST Bioscience we synthesized that sequence and further pieced together with 65777 backbone in our lab.

References

Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature. 2019 Oct 21;576.