Difference between revisions of "Part:BBa K4830006:Design"
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===Design Notes=== | ===Design Notes=== | ||
The design of HEK3 pegRNA includes the U6 promoter, spacer, guide RNA scaffold, rttpbs and polyA tail. | The design of HEK3 pegRNA includes the U6 promoter, spacer, guide RNA scaffold, rttpbs and polyA tail. | ||
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===Source=== | ===Source=== | ||
Latest revision as of 17:01, 10 October 2023
HEK3 pegRNA
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The design of HEK3 pegRNA includes the U6 promoter, spacer, guide RNA scaffold, rttpbs and polyA tail.
Source
The original HEK3 pegRNA design containing spacer and rttpbs sequence was reported in Prime Editor 2 paper cited in References. With the help of TWIST Bioscience we synthesized those and other necessary sequences, and further combined with 65777 backbone in our lab.
References
Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature. 2019 Oct 21;576.