Difference between revisions of "Part:BBa K4830005:Design"
Aruzhan001 (Talk | contribs) |
|||
| Line 8: | Line 8: | ||
===Design Notes=== | ===Design Notes=== | ||
The design of EMX1 ngRNA includes the U6 promoter, spacer, guide RNA scaffold, and polyA tail. | The design of EMX1 ngRNA includes the U6 promoter, spacer, guide RNA scaffold, and polyA tail. | ||
| − | |||
| − | |||
===Source=== | ===Source=== | ||
| Line 16: | Line 14: | ||
===References=== | ===References=== | ||
| + | Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature. 2019 Oct 21;576. | ||
Latest revision as of 16:43, 10 October 2023
EMX1 ngRNA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The design of EMX1 ngRNA includes the U6 promoter, spacer, guide RNA scaffold, and polyA tail.
Source
The original EMX1 ngRNA design containing spacer sequence was reported in Prime Editor 2 paper cited in References. With the help of TWIST Bioscience we synthesized that sequence and further pieced together with 65777 backbone in our lab.
References
Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature. 2019 Oct 21;576.