Difference between revisions of "Part:BBa K4743034"

Latest revision as of 15:21, 10 October 2023

Astaxanthin production set

The engineered Kluyveromyces marxianus exhibits a red coloration as a result of successful genetic modification, indicating the accumulation of astaxanthin within the cells. This color change serves as a visual confirmation of the genetic engineering success. Yeast cells reproduce through a process known as budding, wherein some newly formed buds may still be attached to the parent cell. While these buds may lack the desired genes, they can survive the antibiotic selection process due to the antibiotic resistance conferred by the engineered parent cell. It was observed that the cell sizes within the culture vary, and not all of them display the red coloration, suggesting that not all cells in the culture contain the desired genes.

To address this variation and further refine the culture, a three-generation streak selection method was employed, taking advantage of the easily observable red phenotype in the engineered Kluyveromyces marxianus.

Overall metabolic pathway of Astaxanthin

Phenotyping

Quantitative check

References

1.Jin, J., Wang, Y., Yao, M., Gu, X., Li, B., Liu, H., Ding, M., Xiao, W., & Yuan, Y. (2018). Astaxanthin overproduction in yeast by strain engineering and new gene target uncovering. Biotechnology for biofuels, 11, 230. https://doi.org/10.1186/s13068-018-1227-4

2.Chang, J. J., Ho, C. Y., Ho, F. J., Tsai, T. Y., Ke, H. M., Wang, C. H., Chen, H. L., Shih, M. C., Huang, C. C., & Li, W. H. (2012). PGASO: A synthetic biology tool for engineering a cellulolytic yeast. Biotechnology for biofuels, 5(1), 53. https://doi.org/10.1186/1754-6834-5-53

Sequence and Features