Difference between revisions of "Part:BBa K4960023"
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| − | <partinfo> | + | <partinfo>BBa_K4960023This part including the packaging domain of PVC and the gene encoding protein Cre and polypeptide HiBiT. Cre is a 38kDa protein composed of 343 amino acids, with 4 subunits and 2 domains: a larger carboxyl (C-terminal) domain and a smaller amino (n-terminal) domain. It is a site-specific DNA recombination enzyme that can specifically recognize loxp sites and mediate sequence deletion or recombination between loxp sites. The sequence of loxp is as follows: |
| + | 5' - ATAACTTCGTATA - GCATACAT - TATACGAAGTTAT - 3' | ||
| + | 3' - TATTGAAGCATAT - CGTATGTA - ATATGCTTCAATA - 5' | ||
| + | HiBiT is a polypeptide tag, can be linked to any protein. | ||
| + | This part can achieve the purpose of mediating Cre protein injection into target cells and gene editing at specific loci in target cells.</partinfo> | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 15:18, 10 October 2023
No part name specified with partinfo tag.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 599
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 285
- 1000COMPATIBLE WITH RFC[1000]