Difference between revisions of "Part:BBa K4830000"
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===Characterization=== | ===Characterization=== | ||
The pegRNA in combination with ngRNA are used to test the efficiency of the Prime Editor containing the alternative Reverse Trancriptases, by changing nucleotide at the RNF2 target site from A to G (T to C). The plasmids containing PE, pegRNA and ngRNA were trasfected into HEK293T cells, and editing efficiency was evaluated 72hr after transfection using Sanger sequencing. | The pegRNA in combination with ngRNA are used to test the efficiency of the Prime Editor containing the alternative Reverse Trancriptases, by changing nucleotide at the RNF2 target site from A to G (T to C). The plasmids containing PE, pegRNA and ngRNA were trasfected into HEK293T cells, and editing efficiency was evaluated 72hr after transfection using Sanger sequencing. | ||
Revision as of 12:13, 10 October 2023
RNF pegRNA
RNF2 pegRNA - prime editing guide RNA targeting the gene encoding Ring Finger Protein 2 (RNF), and introduces A to G (T to C) base inversion
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]