Difference between revisions of "Part:BBa K4808011"

 
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<partinfo>BBa_K4808011 short</partinfo>
 
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pEcCas plasmid carry cas 9 protein which can cut the target gene.  
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pEcCas plasmid carry the cas 9 protein which can cut the target gene.  
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===Usage and Biology===
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    <h2><b>Characterization</b></h2>
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<span class='h3bb'>Sequence and Features</span>
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    <p>pEcCas plasmid play an important role in our CRISPR-CAS 9 knocking out experiment. </p >
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<partinfo>BBa_K4808011 SequenceAndFeatures</partinfo>
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https://static.igem.wiki/teams/4808/wiki/1280x1280.png
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    <p> Step1: Transformation of the pEcCas plasmid(carry cas9 protein) into E. coli AIS-0 (CICC20905). Step2:  construction of the pTarget plasmid and donor DNA. Step3:  transformation of pTarget plasmid and donor DNA into the AIS-0 with pEsCas inserted. Step4: Incubation of the transformed strains. </p >
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K4808011 parameters</partinfo>
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<partinfo>BBa_K4808003 parameters</partinfo>
 
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Revision as of 11:08, 10 October 2023

pEcCas

pEcCas plasmid carry the cas 9 protein which can cut the target gene.

Characterization

pEcCas plasmid play an important role in our CRISPR-CAS 9 knocking out experiment.

1280x1280.png

Step1: Transformation of the pEcCas plasmid(carry cas9 protein) into E. coli AIS-0 (CICC20905). Step2: construction of the pTarget plasmid and donor DNA. Step3: transformation of pTarget plasmid and donor DNA into the AIS-0 with pEsCas inserted. Step4: Incubation of the transformed strains.