Difference between revisions of "Part:BBa K4830000"
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| + | RNF2 pegRNA - prime editing guide RNA targeting the gene encoding Ring Finger Protein 2 (RNF), and introduces A to G (T to C) base inversion | ||
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| + | <!-- Add more about the biology of this part here | ||
| + | ===Usage and Biology=== | ||
Prime editing is an innovative technology for genome editing that enables the installation of the wide spectrum of gene modifications such as 12 possible base-to-base conversions, small insertions, and deletions, without requiring double-stranded breaks or donor DNA templates. This technology provides high versatility and target specificity, offering the potential to revolutionize medicine by providing novel tools for treating genetic diseases. Prime editing relies on specialized prime editors, which usually consist of reverse transcriptase enzyme fused to nickase Cas9, and prime editing guide RNA containing a spacer that specifies the target site. It also includes a scaffold and 3’ extension containing a primer binding site (PBS) and an RT template encoding the desired edit. | Prime editing is an innovative technology for genome editing that enables the installation of the wide spectrum of gene modifications such as 12 possible base-to-base conversions, small insertions, and deletions, without requiring double-stranded breaks or donor DNA templates. This technology provides high versatility and target specificity, offering the potential to revolutionize medicine by providing novel tools for treating genetic diseases. Prime editing relies on specialized prime editors, which usually consist of reverse transcriptase enzyme fused to nickase Cas9, and prime editing guide RNA containing a spacer that specifies the target site. It also includes a scaffold and 3’ extension containing a primer binding site (PBS) and an RT template encoding the desired edit. | ||
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RNF2 also known as Ring Finger Protein 2 is a gene encoding for protein, that is part of Polycomb group (PcG) of proteins. This complex is significant in the transcription repression of various genes involved in development and cell proliferation. Luo-Schoch-Yamamoto Syndrome and Non-Specific Syndromic Intellectual Disability are associated with RNF2. | RNF2 also known as Ring Finger Protein 2 is a gene encoding for protein, that is part of Polycomb group (PcG) of proteins. This complex is significant in the transcription repression of various genes involved in development and cell proliferation. Luo-Schoch-Yamamoto Syndrome and Non-Specific Syndromic Intellectual Disability are associated with RNF2. | ||
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| + | ===Characterization=== | ||
| + | The pegRNA in combination with ngRNA are used to test the efficiency of the Prime Editor containing the alternative Reverse Trancriptases, by changing nucleotide at the RNF2 target site from A to G (T to C). The plasmids containing PE, pegRNA and ngRNA were trasfected into HEK293T cells, and editing efficiency was evaluated 72hr after transfection using Sanger sequencing. | ||
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Revision as of 12:11, 10 October 2023
RNF pegRNA
RNF2 pegRNA - prime editing guide RNA targeting the gene encoding Ring Finger Protein 2 (RNF), and introduces A to G (T to C) base inversion
Characterization
The pegRNA in combination with ngRNA are used to test the efficiency of the Prime Editor containing the alternative Reverse Trancriptases, by changing nucleotide at the RNF2 target site from A to G (T to C). The plasmids containing PE, pegRNA and ngRNA were trasfected into HEK293T cells, and editing efficiency was evaluated 72hr after transfection using Sanger sequencing.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]