Difference between revisions of "Part:BBa K4830001:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | The design of RNF2 ngRNA includes the U6 promoter, spacer, guide RNA scaffold, and polyA tail. | |
===Source=== | ===Source=== | ||
− | + | The original RNF2 ngRNA design containing spacer sequence was reported in Prime Editor 2 paper cited in References. We used that sequence and with the help of and TWIST Bioscience synthesized it, which was further pieced together with 65777 backbone in our lab. | |
− | + | ||
===References=== | ===References=== | ||
+ | .Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature. 2019 Oct 21;576. |
Revision as of 16:05, 10 October 2023
RNF ngRNA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The design of RNF2 ngRNA includes the U6 promoter, spacer, guide RNA scaffold, and polyA tail.
Source
The original RNF2 ngRNA design containing spacer sequence was reported in Prime Editor 2 paper cited in References. We used that sequence and with the help of and TWIST Bioscience synthesized it, which was further pieced together with 65777 backbone in our lab.
References
.Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, et al. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature. 2019 Oct 21;576.