Difference between revisions of "Part:BBa K4743005"
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<center>Figure 1. The Matabolic pathway of NMN production.</center> | <center>Figure 1. The Matabolic pathway of NMN production.</center> | ||
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+ | <span class='h3bb'><big>'''Length checking of NadV sequence'''</big></span> | ||
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+ | <html><img style="width:800px" src="https://static.igem.wiki/teams/4743/wiki/folder-for-parts/pnuc-nadv-prsa-length.png" </html> | ||
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+ | <center>Figure 2. The legnth of NadV sequence .</center> | ||
===References=== | ===References=== |
Latest revision as of 15:31, 10 October 2023
NadV+ADH1 terminator with adapter
NadV is a nicotinamide phosphoribosyltransferase that make Nicotinamide+ Prpp into NMN.
Overall metabolic pathway of NMN
NadV is involved in the pathway of Astaxanthin production in PTSH-Taiwan's project.
Length checking of NadV sequence
References
1. Martin, P. R., Shea, R. J., & Mulks, M. H. (2001). Identification of a plasmid-encoded gene from Haemophilus ducreyi which confers NAD independence. Journal of bacteriology, 183(4), 1168–1174. https://doi.org/10.1128/JB.183.4.1168-1174.2001
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1689
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1689
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1689
Illegal BglII site found at 515
Illegal BamHI site found at 16
Illegal BamHI site found at 367
Illegal BamHI site found at 975 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1689
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1689
Illegal AgeI site found at 1702 - 1000COMPATIBLE WITH RFC[1000]