Difference between revisions of "Part:BBa K4759204"
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Revision as of 07:16, 10 October 2023
T7-RBS4-SUMO-linker-OleP
we add a solubilization-promoting tag at the N-end of the P450 enzyme, and in the selected six solubilization-promoting tags (SUMO, GST, MBP, TF, TRX, Nus)
Usage and Biology
Next, we add a solubilization-promoting tag at the N-end of the P450 enzyme, and in the selected six solubilization-promoting tags (SUMO, GST, MBP, TF, TRX, Nus), MBP and TF can significantly improve the solubility of Olep protein, but the enzyme activity of Olep is reduced, and the catalytic efficiency is lower than strain without solubilizing tag.
Fig1: The application of protein tags Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 295
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 295
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 295
Illegal BglII site found at 184 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 295
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 295
Illegal AgeI site found at 420
Illegal AgeI site found at 518 - 1000COMPATIBLE WITH RFC[1000]