Difference between revisions of "Part:BBa K4759201"

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<partinfo>BBa_K4759201 short</partinfo>
 
<partinfo>BBa_K4759201 short</partinfo>
  
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Since the CYP107 family is a typical three-component P450 enzyme that requires a pair of redox partner to transfer electrons from the electron donor NAD(P)H to the P450 enzyme activity center, ferredoxin reductase CamA and ferredoxin CamB are selected as redox partner. The codon-optimized camA gene and camB gene are synthesized, and they are subcloned to the plasmid pACYCDuet to obtain the recombinant plasmid pACYCDuet-camA-camB
 
  
 
===Design ===
 
===Design ===

Revision as of 06:57, 10 October 2023


T7-RBS1-camA-RBS2-camB


Design

codon-optimized

Usage and Biology

the CYP107 family is a typical three-component P450 enzyme that requires a pair of redox partner to transfer electrons from the electron donor NAD(P)H to the P450 enzyme activity center, ferredoxin reductase CamA and ferredoxin CamB are selected as redox partner. The codon-optimized camA gene and camB gene are synthesized, and they are subcloned to the plasmid pACYCDuet to obtain the recombinant plasmid pACYCDuet-camA-camB Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1366
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1366
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1366
    Illegal BamHI site found at 1360
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1366
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1366
    Illegal AgeI site found at 124
    Illegal AgeI site found at 418
  • 1000
    COMPATIBLE WITH RFC[1000]