Difference between revisions of "Part:BBa K4885012"
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<partinfo>BBa_K4885012 short</partinfo> | <partinfo>BBa_K4885012 short</partinfo> | ||
| − | + | This part is responsible for the expression of adhE2 gene with Ptkt promotor. adhE2 gene is derived from Clostridium acetobutylicum. Ptkt is a native promoter that drives the expression of transketolase (tkt) gene in C. tyrobutyricum. This part consists of Ptkt sequence (BBa_K4886005), adhE2 sequence (BBa_K1462060), and terminator sequence (BBa_K3585002). adhE2 gene encodes alcohol/aldehyde bifunctional dehydrogenase. Its role is to convert acyl-CoA to aldehyde then to alcohol in two reductive steps using NADH as cofactor. | |
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Revision as of 07:19, 10 October 2023
Ptkt-adhE2
This part is responsible for the expression of adhE2 gene with Ptkt promotor. adhE2 gene is derived from Clostridium acetobutylicum. Ptkt is a native promoter that drives the expression of transketolase (tkt) gene in C. tyrobutyricum. This part consists of Ptkt sequence (BBa_K4886005), adhE2 sequence (BBa_K1462060), and terminator sequence (BBa_K3585002). adhE2 gene encodes alcohol/aldehyde bifunctional dehydrogenase. Its role is to convert acyl-CoA to aldehyde then to alcohol in two reductive steps using NADH as cofactor.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]