Difference between revisions of "Part:BBa K203101"
Line 3: Line 3:
  
 
This part is a protein domain of the Sar-1 GTPase known to localize to the ER membrane. It is very convenient as a localization signal to the ER for proteins to which it is fused.
 
This part is a protein domain of the Sar-1 GTPase known to localize to the ER membrane. It is very convenient as a localization signal to the ER for proteins to which it is fused.
−
 
−
[[Image:ER2.jpg]]
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 14: Line 12:
  
  
−
<!-- Uncomment this to enable Functional Parameter display
 
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K203101 parameters</partinfo>
 
<partinfo>BBa_K203101 parameters</partinfo>
 +
 +
Shown to localize proteins to the ER. An expression device consisting of [[Part:BBa_I712004]] (CMV) [[Part:BBa_K203103]] (GFP), [[Part:BBa_K203101]] (Sar-1) and [[Part:BBa_K203102]] (terminator) was assembled for this characterization.
 +
[[Image:ER2.jpg]]
 
<!-- -->
 
<!-- -->

Revision as of 08:44, 20 October 2009

ER membrane targeting (C-terminal)

This part is a protein domain of the Sar-1 GTPase known to localize to the ER membrane. It is very convenient as a localization signal to the ER for proteins to which it is fused.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 133
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 133
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 133
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

n/aER membrane targeting (C-terminal)

Shown to localize proteins to the ER. An expression device consisting of Part:BBa_I712004 (CMV) Part:BBa_K203103 (GFP), Part:BBa_K203101 (Sar-1) and Part:BBa_K203102 (terminator) was assembled for this characterization. ER2.jpg