Difference between revisions of "Part:BBa K3717006:Experience"
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We used this part as a control of our experiment. We were able to express this part using a standard IPTG induction protocol. Cells were inducted with 0.1 mM IPTG and incubated for 18 hours overnight at 26 °C. Under our protocol, we were able to see significant overproduction of proteins. Most of which were in the pellet (unsoluble extract). We qualitatively tested the enzyme and detected an α-galactosidase activity. See https://2023.igem.wiki/ulaval/ for details. | We used this part as a control of our experiment. We were able to express this part using a standard IPTG induction protocol. Cells were inducted with 0.1 mM IPTG and incubated for 18 hours overnight at 26 °C. Under our protocol, we were able to see significant overproduction of proteins. Most of which were in the pellet (unsoluble extract). We qualitatively tested the enzyme and detected an α-galactosidase activity. See https://2023.igem.wiki/ulaval/ for details. | ||
| − | https://static.igem.wiki/teams/4726/wiki/results/results- | + | https://static.igem.wiki/teams/4726/wiki/results/sds-page-purif-culot-lysat-ft-igem.png |
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| + | <b>Figure 1</b>. SDS-PAGE electrophoresis of lysis extracts. | ||
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| + | https://static.igem.wiki/teams/4726/wiki/results/sds-page-purif-wash-elution-igem.png | ||
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| + | <b>Figure 2</b>.SDS-PAGE electrophoresis of purification steps. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 01:46, 10 October 2023
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K3717006
iGEM ULaval 2023
We used this part as a control of our experiment. We were able to express this part using a standard IPTG induction protocol. Cells were inducted with 0.1 mM IPTG and incubated for 18 hours overnight at 26 °C. Under our protocol, we were able to see significant overproduction of proteins. Most of which were in the pellet (unsoluble extract). We qualitatively tested the enzyme and detected an α-galactosidase activity. See https://2023.igem.wiki/ulaval/ for details.
Figure 1. SDS-PAGE electrophoresis of lysis extracts.
Figure 2.SDS-PAGE electrophoresis of purification steps.
User Reviews
UNIQ5e0577322a8913a0-partinfo-00000000-QINU UNIQ5e0577322a8913a0-partinfo-00000001-QINU