Difference between revisions of "Part:BBa K4743032"
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<center>Figure 1. The Matabolic pathway and map of HMB and KIC production.</center> | <center>Figure 1. The Matabolic pathway and map of HMB and KIC production.</center> | ||
+ | <html><img style="width:800px" src="https://static.igem.wiki/teams/4743/wiki/folder-for-parts/hmb-hplc-peak-igem-update.png" </html> | ||
+ | <center>Figure 2. The quantitative analysis for HMB.</center> | ||
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Revision as of 06:25, 10 October 2023
Bacillus subtilis HMB production set
This part is uesd for the production of HMB and KIC in Bacillus subtilis
Overall metabolic pathway of HMB
We use the gene HPD and LAD to let B.Subtilis to produce HMB and KIC
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 511
Illegal XhoI site found at 193 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2162
- 1000COMPATIBLE WITH RFC[1000]