Difference between revisions of "Part:BBa K4726000:Design"

(→‎Source)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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This gene originates from <i>Flavonifractor plautii</i>
+
The clone this gene onto our expression vector (pET-3a) we added NdeI site in 5' and BamHI site in 3' of the gene. We added TATATA sequences before NdeI site and after BamHI site to help with the restriction digestion.
  
 
===Source===
 
===Source===

Revision as of 23:00, 9 October 2023


N-acetyl-α-D-galactosamine deacetylase_Flavonifractor plautii


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 865
    Illegal EcoRI site found at 1411
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 865
    Illegal EcoRI site found at 1411
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 865
    Illegal EcoRI site found at 1411
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 865
    Illegal EcoRI site found at 1411
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 865
    Illegal EcoRI site found at 1411
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1894
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The clone this gene onto our expression vector (pET-3a) we added NdeI site in 5' and BamHI site in 3' of the gene. We added TATATA sequences before NdeI site and after BamHI site to help with the restriction digestion.

Source

This gene originates from Flavonifractor plautii, a bacterium from the human gut microbiota. It encodes a N-acetyl-α-D-galactosamine deacetylase.

References