Difference between revisions of "Part:BBa K4613097"

Revision as of 17:41, 9 October 2023

Plac

IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. This part additionally includes the lac operator(symmetric), which was optimized for tight binding of lac repressor to avoid the leakage of the downstream gene.

Reference

White CE, Winans SC. Identification of amino acid residues of the Agrobacterium tumefaciens quorum-sensing regulator TraR that are critical for positive control of transcription. Mol Microbiol. 2005 Mar;55(5):1473-86. doi: 10.1111/j.1365-2958.2004.04482.x. PMID: 15720554.

Fuqua C, Winans SC. Conserved cis-acting promoter elements are required for density-dependent transcription of Agrobacterium tumefaciens conjugal transfer genes. J Bacteriol. 1996 Jan;178(2):435-40. doi: 10.1128/jb.178.2.435-440.1996. PMID: 8550463; PMCID: PMC177675.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 60
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 60
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 60
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 60
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 60
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription.
-This part additionally includes the <em>lac<em> operator(symmetric), which was optimized for tight binding of <em>lac<em> repressor to avoid the leakage of the downstream gene.
+This part additionally includes the <em>lac</em> operator(symmetric), which was optimized for tight binding of <em>lac</em> repressor to avoid the leakage of the downstream gene.
+==== Reference ====
+#White CE, Winans SC. Identification of amino acid residues of the Agrobacterium tumefaciens quorum-sensing regulator TraR that are critical for positive control of transcription. Mol Microbiol. 2005 Mar;55(5):1473-86. doi: 10.1111/j.1365-2958.2004.04482.x. PMID: 15720554.
+Fuqua C, Winans SC. Conserved cis-acting promoter elements are required for density-dependent transcription of Agrobacterium tumefaciens conjugal transfer genes. J Bacteriol. 1996 Jan;178(2):435-40. doi: 10.1128/jb.178.2.435-440.1996. PMID: 8550463; PMCID: PMC177675.
 <!-- Add more about the biology of this part here