Difference between revisions of "Part:BBa K4664002:Design"
Yiming Dong (Talk | contribs) (→Design Notes) |
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===Design Notes=== | ===Design Notes=== | ||
− | Padlock Padlock-miR-223 is the corresponding padlock chosen for the biomarker miRNA-223. | + | Padlock Padlock-miR-223 is the corresponding padlock chosen for the biomarker miRNA-223. The padlock probe for RCA is engineered with a split ligation module for target miRNA binding and a CRISPR–Cas12a detection module whose complementary sequence activates a Cas12a–crRNA complex. miRNA sequences are extracted from a sample, annealed with the padlock and then added to a reaction tube containing the enzymes, reporter and other reagents. |
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===Source=== | ===Source=== |
Latest revision as of 16:13, 9 October 2023
Padlock-miR-223
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Padlock Padlock-miR-223 is the corresponding padlock chosen for the biomarker miRNA-223. The padlock probe for RCA is engineered with a split ligation module for target miRNA binding and a CRISPR–Cas12a detection module whose complementary sequence activates a Cas12a–crRNA complex. miRNA sequences are extracted from a sample, annealed with the padlock and then added to a reaction tube containing the enzymes, reporter and other reagents.
Source
Sequence synthesis through a CRO company.