Difference between revisions of "Part:BBa K4863000"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | Carbonic anhydrases (CAs) can catalyze the biochemical process necessary for the biotechnological method of microbially induced calcite precipitation (MICP), which has gained increasing attention due to its potential in creating calcium carbonate precipitation for increasing the compressive strength of concrete. CAs catalyze the conversion of carbon dioxide to bicarbonate ions, and the ions can react with CaCl to form calcium carbonate deposition, which acts as an adhesive for increasing and maintaining compressive strength of concrete. hpCA, the α-carbonic anhydrase isolated from Helicobacter pylori 26695, has optimal performance among the CAs characterized so far for expression in E. Coli. Additionally, it has been shown that surface display is necessary for maintaining the stability of the enzyme (Zhu et al., 2022). | ||
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Revision as of 15:11, 9 October 2023
α-carbonic anhydrase from Helicobacter pylori 26695 (hpCA)
α-carbonic anhydrases are metalloenzymes responsible for catalyzing the interconversion of carbon dioxide (CO2) to bicarbonate ions (HCO3−). hpCA is an α-carbonic anhydrase isolated from the bacterium Helicobacter pylori 26695, codon optimized for expression in E. Coli BL21(DE3) (Zhu et al., 2022).
In our work, CA is expressed in E. Coli and displayed on the surface of Synechocystis PCC6803 for converting carbon dioxide into bicarbonate ions, which then forms calcium carbonate (CaCO3) precipitates, thus achieving biocrystallization and the production of biological concrete.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 414
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]