Difference between revisions of "Part:BBa K203107"
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| − | |[[Image:HD09_1110_c-jun2.png|thumb|none|350px|'''BBa_K203106 is a natural HMG CoA synthase promoter induced | + | |[[Image:HD09_1110_c-jun2.png|thumb|none|350px|'''BBa_K203106 is a natural HMG CoA synthase promoter induced more than 100% by Epidermal Growth Factor'''. The GFP flourescence of the c-Jun promoter (in REU) was measured by flow cytometry once (à three replicates).]] |
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Revision as of 11:12, 19 October 2009
c-Jun promoter (growth factor regulated)
This part is a natural c-Jun promoter and when placed upstream of other genes could induce their transcription in response to Epidermal Growth Factor to the medium.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 398
Illegal BamHI site found at 392 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 110
Functional Parameters
| n/a | c-Jun promoter (growth factor regulated) |
The natural c-Jun promoter was induced by Epidermal Growth Factor (1:10000 in DMEM++)(more details: see Cell Culture Notebook) in HeLa cells. The GFP expression level was determined in REU by flow cytometry (1 experiment, 3 replicates) 3 hours after induction. For more natural promoters look at Part:BBa_K203105 and Part:BBa_K203106.
